human embryonic lung fibroblast cell line mrc 5  (ATCC)

Journal: Materials Today Bio

Article Title: Polystyrene nanoplastics-induced lung epithelial cells ferroptosis promotes pulmonary fibrosis via YY1/FTL axis

doi: 10.1016/j.mtbio.2025.102738

Figure Lengend Snippet: PS-NPs-Induced Ferroptosis in BEAS-2B Cells and Paracrine Fibroblast Activation: (A–B) Western blot of GPX4 and FTL in BEAS-2B cells after 24 h exposure to PS-NPs at 0, 50, 100, and 200 μg/ml (C–D) Time-course of GPX4 and FTL expression in cells treated with 200 μg/ml PS-NPs for 0, 3, 6, 12, and 24 h. (E) Transmission electron microscopy images of control and PS-NPs treated BEAS-2B cells showing characteristic mitochondrial shrinkage. (F–G) Restoration of GPX4 following co-treatment with Fer-1. (H) C11-BODIPY581/591 fluorescence showing Fer-1–mediated attenuation of lipid peroxidation. (I) FerroOrange staining demonstrating reduction of labile Fe 2+ upon Fer-1 co-incubation. (J–O) COL1 and α-SMA mRNA (qPCR) and protein (western blot) levels in MRC-5 fibroblasts after 24 h treatment with conditioned medium from BEAS-2B cells exposed to PS-NPs ± Fer-1. Statistical significance is denoted as *p < 0.05 and **p < 0.01.

Article Snippet: The human bronchial epithelial cell (BEAS-2B) and human embryonic lung fibroblast (MRC-5) were commercially purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Activation Assay, Western Blot, Expressing, Transmission Assay, Electron Microscopy, Control, Fluorescence, Staining, Incubation

Journal: Materials Today Bio

Article Title: Polystyrene nanoplastics-induced lung epithelial cells ferroptosis promotes pulmonary fibrosis via YY1/FTL axis

doi: 10.1016/j.mtbio.2025.102738

Figure Lengend Snippet: YY1 Silencing Reverses PS-NPs–Induced Ferroptosis and Paracrine Fibrogenesis: (A–C) Western blots showing restoration of GPX4 protein in BEAS-2B cells after YY1 knockdown and 24 h PS-NPs exposure. (D–E) C11-BODIPY581/591 fluorescence demonstrating reduced lipid peroxidation upon YY1 silencing. (F) FerroOrange staining indicating decreased labile Fe 2+ accumulation after YY1 knockdown. (G–H) Double immunofluorescence staining of YY1 with the epithelial marker SPC and the myofibroblast marker α-SMA in lung sections from PS-NPs-treated mice. (I–L) Western blot and qRT-PCR analyses of COL1 and α-SMA in MRC-5 fibroblasts treated for 24 h with conditioned medium from BEAS-2B cells exposed to PS-NPs ± YY1 silencing. Statistical significance is denoted as *p < 0.05 and **p < 0.01.

Article Snippet: The human bronchial epithelial cell (BEAS-2B) and human embryonic lung fibroblast (MRC-5) were commercially purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Western Blot, Knockdown, Fluorescence, Staining, Double Immunofluorescence Staining, Marker, Quantitative RT-PCR